Last data update: May 13, 2024. (Total: 46773 publications since 2009)
Records 1-3 (of 3 Records) |
Query Trace: Araujo AC[original query] |
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Antibody- and genome-based identification of recent HCV infection.
Araujo AC . Antivir Ther 2012 17 1459-64 The diagnosis of recent HCV infection remains challenging due to the absence of serological markers specific to the early phase of infection. Clinical follow-up and seroconversion to anti-HCV immunoglobulin (Ig)G, detection of viral RNA and changes in levels of blood biomarkers associated with liver pathology provide circumstantial evidence of recent HCV infection. Studies based on anti-HCV IgG avidity, antigen-specific antibody profiling, HCV viral load fluctuations and signature changes in the HCV genome show potential to discriminate recent from persistent HCV infection. These markers require further evaluation and would necessitate use of samples from infected people originating from broad clinical and epidemiological contexts. |
Laboratory diagnostics for hepatitis C virus infection
Kamili S , Drobeniuc J , Araujo AC , Hayden TM . Clin Infect Dis 2012 55 Suppl 1 S43-8 Identification of prevalent infection by hepatitis C virus (HCV) is based serologically on detecting anti-HCV immunoglobulin G, using immunoassays, immunoblot assays, and, more recently, immunochromatography-based rapid tests. None discriminate between active and resolved HCV infection. Tests for detecting HCV RNA identify active HCV infection but are costly. Serologic assays for HCV antigens have been developed and show potential for diagnosis of active HCV infection, and their performance characteristics are undergoing evaluation. The diagnosis of acute HCV infection without the demonstration of seroconversion remains elusive. |
Distinguishing acute from chronic Hepatitis C Virus (HCV) infection based on antibody reactivities to specific HCV structural and non-structural proteins
Araujo AC , Astrakhantseva IV , Fields HA , Kamili S . J Clin Microbiol 2010 49 (1) 54-7 Currently available serological assays for detection of antibodies to hepatitis C virus (HCV) cannot reliably discriminate acute from chronic HCV infection. We developed a multiplexed, flow-cytometric microsphere immunoassay to measure anti-HCV-IgG reactivities to core, NS3, NS4 and NS5 HCV recombinant proteins and applied it to 99 serum samples from 24 anti-HCV seroconvertors and 141 anti-HCV-IgG and HCV-RNA-positive plasma specimens from chronically infected people. Differences in the geometric means or mean of signal/cut-off ratios between the two sample sets were statistically significant for all the antigens tested. A multivariate logistic regression model correctly classified the samples in two groups with a cross-validation accuracy of 90.8% for the acute group and 97.2% for the chronic group. The immunoassay described has potential to distinguish acute from chronic HCV infection. |
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